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TRANSFAC
PROSIT: Online Pseudorotation Tool Version 2. Pseudo-Rotational Online Service and Interactive tool (PROSIT). Calculates, and displays in tabular format, the pseudorotation parameters (P, chi, numax) and other useful information for 3D structures of nucleosides, nucleotides and analogs thereof, as well as for DNA and RNA single and double strands, whether by themselves or complexed with a. TRANSFAC ® is the database of eukaryotic transcription factors, their genomic binding sites and DNA-binding profiles. Dating back to a very early compilation, it has been carefully maintained and curated since then and became the gold standard in the field, which can be made use of when applying the geneXplain platform. An online drinking game that will get you very very drunk. RIAU ONLINE, PINGGIR - Seorang pria, berinisial MAB, di Kecamatan Tualang Muandau, Kabupaten Bengkalis, Riau dengan beringas menganiaya anak tirinya hingga tewas. Penyebabnya sederhana, bocah mungil bernama AR yang belum genap berusia dua tahun itu menangis histeris usai dipukuli pria 30 tahun.
The gold standard in the area of transcriptional regulation.
In particular its library of positional weight matrices is a unique collection of DNA-binding models, suitable for a comprehensive analysis of genomic sequences for potential transcription factor binding sites (TFBSs).
You can use TRANSFAC® as encyclopedia of transcriptional regulation, or as a tool to identify potential TFBSs. The latter can be done with the proven tool MatchTM, or with any of the respective modules in the geneXplain platform.
| Cheap Thrills | |
|---|---|
| Directed by | E. L. Katz |
| Produced by |
|
| Written by | |
| Starring | |
| Music by | Mads Heldtberg |
| Cinematography | |
| Edited by | Brody Gusar |
Production companies | |
| Distributed by | Drafthouse Films |
Release date |
|
| 85 minutes | |
| Country | United States |
| Language | English |
| Budget | $100,000[1] |
| Box office | $59,424 (US)[2] |
Cheap Thrills is a 2013 black comedythriller film directed by E. L. Katz in his directorial debut. It premiered at South by Southwest (SXSW) on March 8, 2013, and was acquired by Drafthouse Films and Snoot Entertainment. It was released on March 24, 2014, in the United States.
Plot[edit]
Craig (Pat Healy) is an auto mechanic who loses his job. He is unable to pay his rent and after seeing the eviction sign, he goes to a dive bar, where he meets an old friend from high school, Vince (Ethan Embry). After their reunion, they meet a rich couple, Colin and Violet (David Koechner and Sara Paxton), who appear friendly and benign initially, and after becoming aware of Craig's dire financial situation, offer them money in return for completing certain tasks to entertain Violet, as it is her birthday.
As the first task, Colin offers fifty dollars to whoever between Craig and Vince can drink a shot he pours first. From there, the danger of the tasks escalates along with the payout. The dares result in a bouncer confronting Craig, who is offered five hundred dollars by Colin to hit him first. He does so and is knocked out. When he comes to, he realizes he been brought to the home of Colin and Violet. Tensions between Craig and Vince begin to emerge when they compete with each other in a breath-holding contest and Vince punches Craig in the stomach to prevent him from winning so that he may claim the prize money himself. Another dare between Colin and Vince involves Vince urinating on Craig's shoes. When Craig angrily goes to clean up in the bathroom, Vince accompanies him and they hatch a plan to rob the couple. Vince divulges that there is $250,000, which he instructs Craig to steal.
At knife-point, Vince manages to get Colin to reveal that the safe is unlocked. Craig retrieves the money and returns only to have Colin and Violet turn the tables on them when they disarm Vince. Colin and Violet agree to let 'bygones be bygones' if Craig and Vince promise to behave, and still allow them to take all of the money, but only if they continue to play. Another dare involves Craig having sex with Violet as well as getting $4,500, the amount required to pay for Craig's month's rent. This angers Vince, who views it as an unfair bet, increasing hostilities between the two friends. Humiliated and feeling guilty for cheating on his wife, Craig withdraws from the game and goes home, having earned enough money to delay homelessness for the time being. Violet, who seems to have begun to develop feelings for him, is upset and becomes withdrawn, causing the game to end, as its purpose was to entertain her. Vince, desperate to win some more money, offers to perform anything asked of him. Colin suggests the amputation of his pinkie finger for $25,000. Just as Vince is about to accept, Craig returns to the game, stating that he only temporarily solves his problem with the $4,500 he has earned so far, and offers to the do the same dare for a smaller sum. Vince also goes lower, and they go back and forth until Craig settles for $15,000. Vince cuts Craig's pinkie off, resulting in Craig winning again, which only serves to anger Vince further.
The next challenge involves eating a cooked dead dog, which had died while trying to eat Craig's finger, with the winner who finishes his portion first receiving $50,000. The contest results in a draw, with the money being awarded to whoever eats Craig's finger. Craig wins but he then gets beaten up by an enraged Vince. After being taken outside to calm down by Colin, he suggests that Vince kill Craig for the remaining portion of the $250,000. Vince considers the offer but finds himself unable to kill his friend; he suggests he and Craig leave but is suddenly shot to death by Craig. Colin calls Craig a taxi and he leaves with his winnings in hand. After he has left, Colin pays Violet her money, as the two had made a bet on which friend would kill the other; Colin chose Vince with Violet choosing Craig. The film ends with Craig arriving home, comforting his child, when suddenly the light turns on and his wife appears, staring at Craig covered in blood and at the money strewn all over the room.
Cast[edit]
- Pat Healy as Craig Daniels
- Sara Paxton as Violet
- Ethan Embry as Vince
- David Koechner as Colin
- Amanda Fuller as Audrey Daniels
- Brighton Sharbino as Luann
- Todd Farmer as a security guard
Production[edit]
Cheap Thrills was the first feature-film directed by E. L. Katz. After premiering at South by Southwest, a day-long auction took place for rights to the film in a rare bidding war. Drafthouse Films, partnered with Snoot Entertainment, won the auction. Drafthouse Films planned on releasing the film theatrically on limited screens.[3] It later screened at the Boston Underground Film Festival and the Fantasia International Film Festival.[4] Drafthouse Films released Cheap Thrills on DVD/Blu-ray May 27, 2014 which included a 45-minute documentary called 'Vital Heat: The Making of Cheap Thrills'.
Reception[edit]
On Rotten Tomatoes the film has a score of 89% based on reviews from 97 critics, with an average rating of 6.95/10. The consensus states 'Gleefully nasty and darkly hilarious, Cheap Thrills lives down to its title in the best possible way.'[5]
Joe Leydon, writing for Variety, said that film 'is a thoroughly nasty piece of work, which doubtless will be the strongest selling point for this worst-case scenario about steadily escalating dares and degradations'.[6] John Defore, of The Hollywood Reporter, said that the film is 'one of the most involving works of cinematic misanthropy in years'. Defore compared the film to Indecent Proposal and The Most Dangerous Game.[4] Scott Weinberg, of the online horror website Fearnet, said, 'Cheap Thrills breezes by on a twisted idea, a fantastic cast, and a bunch of ethical quandaries that are both eerily uncomfortable and slyly fascinating at the same time.'[7] Simon Foster of SBS Films said, 'A textbook case of a film that never quite amounts to the sum of its parts, Cheap Thrills is still more than its title suggests and provides dark entertainment for those in the mood.'[8]
References[edit]
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- ^Gilchrist, Todd. 'Cheap Thrills Director E.L. Katz On Small Budgets, Big Ideas And The Horror Of Financial Success'. Forbes. Retrieved June 13, 2014.
- ^'Cheap Thrills'. Box Office Mojo. Retrieved January 27, 2017.
- ^Yamato, Jen (March 11, 2013). 'UPDATE: Drafthouse Films Picks Up 'Cheap Thrills' In First Deal Of SXSW 2013'. Deadline. Retrieved August 28, 2013.
- ^ abDeFore, John (August 6, 2013). 'Cheap Thrills – H2013 Cheap Thrills: Film Review'. The Hollywood Reporter. Retrieved August 28, 2013.
- ^'Cheap Thrills (2014)'. Rotten Tomatoes. Retrieved 1 August 2019.
- ^Leydon, Joe (March 22, 2013). 'Film Review: 'Cheap Thrills''. Variety. Retrieved August 28, 2013.
- ^Weinberg, Scott (March 9, 2013). 'FEARnet Movie Review: 'Cheap Thrills''. Fearnet. Retrieved August 28, 2013.
- ^Foster, Simon. 'Cheap Thrills'. SBS Films. Retrieved August 28, 2013.
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External links[edit]
- Official website – Drafthouse Films
- Cheap Thrills at IMDb
- Cheap Thrills at Rotten Tomatoes